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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC76 Polyclonal antibody (31484-1-AP)

    WB analysis using 31484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of human placenta using 31484-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC76 Polyclonal antibody (31484-1-AP)

    IHC staining of mouse testis using 31484-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC76 Polyclonal antibody (31484-1-AP)

    IF Staining of HeLa using 31484-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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CCDC76 Polyclonal antibody

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31484-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

tRNA [Gm4] methyltransferase, TRMT13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, LNCaP cells
Positive IHC detected inhuman placenta tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31484-1-AP targets CCDC76 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC76 fusion protein Ag34110 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 76
Observed molecular weight54 kDa
GenBank accession numberBC075811
Gene Symbol CCDC76
Gene ID (NCBI) 54482
RRIDAB_3670001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NUP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).

Protocols

Product Specific Protocols
WB protocol for CCDC76 antibody 31484-1-APDownload protocol
IHC protocol for CCDC76 antibody 31484-1-APDownload protocol
IF protocol for CCDC76 antibody 31484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols