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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC47 Polyclonal antibody (32080-1-AP)

    WB analysis using 32080-1-AP (same clone as 32080-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32080-1-AP (CCDC47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC47 Polyclonal antibody (32080-1-AP)

    IHC staining of mouse testis using 32080-1-AP (same clone as 32080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CCDC47 Polyclonal antibody (32080-1-AP)

    IHC staining of mouse cerebellum using 32080-1-AP (same clone as 32080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CCDC47 Polyclonal antibody (32080-1-AP)

    IHC staining of mouse cerebellum using 32080-1-AP (same clone as 32080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32080-1-PBS in a different storage buffer formulation.

CCDC47 Polyclonal antibody, PBS Only

CCDC47 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32080-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32080-1-PBS targets CCDC47 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36570

Product name: Recombinant human CCDC47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-483 aa of BC008905

Sequence: GKNKNSRLAQAWFNTHRELLESNFTLVGDDGTNKEATSTGKLNQENEHIYNLWCSGRVCCEGMLIQLRFLKRQDLLNVLARMMRPVSDQVQIKVTMNDEDMDTYVFAVGTRKALVRLQKEMQDLSEFCSDKPKSGAKYGLPDSLAILSEMGEVTDGMMDTKMVHFLTHYADKIESVHFSDQFSGPKIMQEEGQPLKLPDTKRTLLFTFNVPGSGNTYPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKLTHVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKVKAM

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 47
Calculated molecular weight56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC008905
Gene Symbol CCDC47
Gene ID (NCBI) 57003
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96A33
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Coiled-coil domain containing 47 (CCDC47, also known as THNS, GK001, and MSTP041) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. It also interacts with the ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief (PMID: 28974366). The expression level of CCDC47 is associated with diabetic cardiomyopathy (PMID: 30140426). Its variant would cause trichohepatoneurodevelopmental syndrome (PMID: 38524542).

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