Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, L02 cells, RT-4 cells, mouse heart tissue, mouse lung tissue, rat heart tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32080-1-AP targets CCDC47 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCDC47 fusion protein Ag36570 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 47 |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC008905 |
| Gene Symbol | CCDC47 |
| Gene ID (NCBI) | 57003 |
| RRID | AB_3670185 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96A33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coiled-coil domain containing 47 (CCDC47, also known as THNS, GK001, and MSTP041) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. It also interacts with the ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief (PMID: 28974366). The expression level of CCDC47 is associated with diabetic cardiomyopathy (PMID: 30140426). Its variant would cause trichohepatoneurodevelopmental syndrome (PMID: 38524542).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC47 antibody 32080-1-AP | Download protocol |
| IHC protocol for CCDC47 antibody 32080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |