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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    WB analysis of mouse lung using 24276-1-AP (same clone as 24276-1-PBS)

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CCDC104 Polyclonal antibody (24276-1-AP)

    WB analysis of HEK-293 using 24276-1-AP (same clone as 24276-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC104 Polyclonal antibody (24276-1-AP)

    IF Staining of A431 using 24276-1-AP (same clone as 24276-1-PBS)

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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CCDC104 Polyclonal antibody, PBS Only

CCDC104 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24276-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24276-1-PBS targets CCDC104 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC104 fusion protein Ag17996 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 104
Calculated molecular weight 342 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC010011
Gene Symbol CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G28
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The CCDC104(Coiled-coil domain-containing protein 104) protein, whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.