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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    WB analysis of mouse lung using 24276-1-AP (same clone as 24276-1-PBS)

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CFAP36 Polyclonal antibody (24276-1-AP)

    WB analysis of HEK-293 using 24276-1-AP (same clone as 24276-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of mouse stomach using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of mouse stomach using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of rat lung using 24276-1-AP (same clone as 24276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CFAP36 Polyclonal antibody (24276-1-AP)

    IF Staining of A431 using 24276-1-AP (same clone as 24276-1-PBS)

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

CFAP36 Polyclonal antibody, PBS Only

CFAP36 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24276-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24276-1-PBS targets CFAP36 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17996

Product name: Recombinant human CCDC104 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC010011

Sequence: MAAEEEDEVEWVVESIAGFLRGPDWSIPILDFVEQKCEVFDDEEESKLTYTEIHQEYKELVEKLLEGYLKEIGINEDQFQEACTSPLAKTHTSQAILQPVLAAEDFTIFKAMMVQKNIEMQLQAIRIIQERNGVLPDCLTDGSDVVSDLEHEEMKILREVLRKSKEEYDQEEERKRKKQLSEAKTEEPTVHSSEAAIMNNSQGDGEHFAHPPSEVKMHFANQSIEPLGRKVERSETSSLPQKGLKFPGLEHASIEGPIANLSVLGTEELRQREHYLKQKRDKLMSMRKDMRTKQIQNMEQKGKPTGEVEEMTEKPEMTAEEKQTLLKRRLLAEKLKEEVINK

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 104
Calculated molecular weight 342 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC010011
Gene Symbol CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G28
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CFAP36 (Cilia- and flagella-associated protein 36), also known as CCDC104(Coiled-coil domain-containing protein 104), whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

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