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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    WB analysis of mouse lung using 24276-1-AP

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CFAP36 Polyclonal antibody (24276-1-AP)

    WB analysis of HEK-293 using 24276-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of mouse stomach using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of mouse stomach using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using CFAP36 Polyclonal antibody (24276-1-AP)

    IHC staining of rat lung using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC104 Polyclonal antibody (24276-1-AP)

    IF Staining of A431 using 24276-1-AP

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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CFAP36 Polyclonal antibody

CFAP36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24276-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, HEK-293 cells
Positive IHC detected inmouse stomach tissue, human testis tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24276-1-AP targets CFAP36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFAP36 fusion protein Ag17996 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 104
Calculated molecular weight 342 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC010011
Gene Symbol CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFAP36 (Cilia- and flagella-associated protein 36), also known as CCDC104(Coiled-coil domain-containing protein 104), whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CFAP36 antibody 24276-1-APDownload protocol
IHC protocol for CFAP36 antibody 24276-1-APDownload protocol
IF protocol for CFAP36 antibody 24276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols