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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    WB analysis of mouse lung using 24276-1-AP

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CCDC104 Polyclonal antibody (24276-1-AP)

    WB analysis of HEK-293 using 24276-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC104 Polyclonal antibody (24276-1-AP)

    IHC staining of human testis using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC104 Polyclonal antibody (24276-1-AP)

    IF Staining of A431 using 24276-1-AP

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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CCDC104 Polyclonal antibody

CCDC104 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24276-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCDC104

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, HEK-293 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24276-1-AP targets CCDC104 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC104 fusion protein Ag17996 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 104
Calculated molecular weight 342 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC010011
Gene Symbol CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CCDC104(Coiled-coil domain-containing protein 104) protein, whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CCDC104 antibody 24276-1-APDownload protocol
IHC protocol for CCDC104 antibody 24276-1-APDownload protocol
IF protocol for CCDC104 antibody 24276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols