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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CBX1 Recombinant antibody (83248-4-RR)

    WB analysis of MCF-7 using 83248-4-RR

    WB result of CBX1 antibody (83248-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using CBX1 Recombinant antibody (83248-4-RR)

    WB analysis using 83248-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    IF Staining of HeLa using 83248-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    FC experiment of HeLa using 83248-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83248-4-RR

    Affinity and Kinetic Characterization of 83248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

  • Featured Product
  • KD/KO Validated

CBX1 Recombinant monoclonal antibody

CBX1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-4-RR
Clone No.240075H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240075H6, CBX, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inMCF-7 cells, mouse spleen tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, human kidney tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83248-4-RR targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34901

Product name: Recombinant human CBX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-115 aa of BC002609

Sequence: CPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFAR

相同性解析による交差性が予測される生物種
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
RRIDAB_3670923
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Protocols

Product Specific Protocols
FC protocol for CBX1 antibody 83248-4-RRDownload protocol
IF protocol for CBX1 antibody 83248-4-RRDownload protocol
WB protocol for CBX1 antibody 83248-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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