• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CBX1 Recombinant antibody (83248-4-RR)

    WB analysis using 83248-4-RR (same clone as 83248-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CBX1 Recombinant antibody (83248-4-RR)

    WB analysis of MCF-7 using 83248-4-RR (same clone as 83248-4-PBS)

    WB result of CBX1 antibody (83248-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CBX1 Recombinant antibody (83248-4-RR)

    FC experiment of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83248-4-RR

    Affinity and Kinetic Characterization of 83248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CBX1 Recombinant antibody, PBS Only

CBX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-4-PBS
Clone No.240075H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240075H6, CBX, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83248-4-PBS targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX1 fusion protein Ag34901 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.