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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CAPZB Monoclonal antibody

CAPZB Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F9G2

Cat no : CL488-68190

Synonyms

CAPB, CAPPB, CAPZ, CapZ beta, CAPZB

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-68190

    IF Staining of A431 using CL488-68190

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CAPZB antibody (CL488-68190, Clone: 1F9G2 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-68190

    FC experiment of Jurkat using CL488-68190

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CAPZB (CL488-68190, Clone:1F9G2) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CAPZB Ready-To-Use IHC Kit. CAPZB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68190-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68190 targets CAPZB in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CAPZB fusion protein Ag19373 相同性解析による交差性が予測される生物種
Full Name capping protein (actin filament) muscle Z-line, beta
Calculated molecular weight 277 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC109241
Gene symbol CAPZB
Gene ID (NCBI) 832
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CAPZB antibody CL488-68190Download protocol
FC protocol for CL Plus 488 CAPZB antibody CL488-68190Download protocol
Standard Protocols
Click here to view our Standard Protocols