Validation Data Gallery

Filter: AllWBSample testIHCIF/ICCFC (Intra)Cytometric bead array

WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)
WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
Sample test of MP51351-1
Sample test of MP51351-1, CALD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66693-1-PBS. Detection antibody: 66693-2-PBS.
IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66693-1-Ig (same clone as 66693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51351-1
Cytometric bead array standard curve of MP51351-1, CALD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66693-1-PBS. Detection antibody: 66693-2-PBS. Standard:Ag13676. Range: 0.391-400 ng/mL.

Featured Product
KD/KO Validated

CALD1 Monoclonal antibody, PBS Only (Capture)

CALD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Cat No. 66693-1-PBS

Clone No.1E1C11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Synonyms

Caldesmon, 1E1C11, CAD, caldesmon 1, CDM

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66693-1-PBS targets CALD1 as part of a matched antibody pair:

MP51351-1: 66693-1-PBS capture and 66693-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CALD1 fusion protein Ag13676 相同性解析による交差性が予測される生物種
Full Name	caldesmon 1
Calculated molecular weight	538 aa, 63 kDa
Observed molecular weight	70 kDa~75 kDa
GenBank accession number	BC040354
Gene Symbol	CALD1
Gene ID (NCBI)	800
RRID	AB_2882047
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q05682
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

Sample test of MP51351-1

IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

IHC staining of human colon using 66693-1-Ig (same clone as 66693-1-PBS)

IF Staining of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)

FC experiment of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

Cytometric bead array standard curve of MP51351-1