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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CACYBP Polyclonal antibody (11745-1-AP)

    WB analysis of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CACYBP Polyclonal antibody (11745-1-AP)

    IP experiment of mouse brain using 11745-1-AP (same clone as 11745-1-PBS)

    IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CACYBP Polyclonal antibody (11745-1-AP)

    IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CACYBP Polyclonal antibody (11745-1-AP)

    IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CACYBP Polyclonal antibody (11745-1-AP)

    IF Staining of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CACYBP Polyclonal antibody (11745-1-AP)

    FC experiment of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CACYBP Polyclonal antibody, PBS Only

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11745-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11745-1-PBS targets CACYBP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2340

Product name: Recombinant human CACYBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-228 aa of BC022352

Sequence: MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF

相同性解析による交差性が予測される生物種
Full Name calcyclin binding protein
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
RRIDAB_2070266
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB71
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

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