Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 11745-1-AP
HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11745-1-AP
WB result of CACYBP antibody (11745-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CACYBP transfected HEK-293 cells.
IP experiment of mouse brain using 11745-1-AP
IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human colon cancer using 11745-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11745-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11745-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11745-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CACYBP Polyclonal antibody

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11745-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Calcyclin, S100 alpha 6, calcyclin binding protein, Calcyclin-binding protein, GIG5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

11745-1-AP targets CACYBP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2340 Product name: Recombinant human CACYBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC022352 Sequence: MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF 相同性解析による交差性が予測される生物種
Full Name	calcyclin binding protein
Calculated molecular weight	228 aa, 26 kDa
Observed molecular weight	26-30 kDa
GenBank accession number	BC022352
Gene Symbol	CACYBP
Gene ID (NCBI)	27101
RRID	AB_2070266
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HB71
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

Protocols

Product Specific Protocols
FC protocol for CACYBP antibody 11745-1-AP	Download protocol
IF protocol for CACYBP antibody 11745-1-AP	Download protocol
IHC protocol for CACYBP antibody 11745-1-AP	Download protocol
IP protocol for CACYBP antibody 11745-1-AP	Download protocol
WB protocol for CACYBP antibody 11745-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Cancer Res MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET Authors - Ankit Srivastava View Article
human	WB	Theranostics CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation. Authors - Yi-Fan Lian KD Validated View Article
human	WB,IHC,IF	J Exp Clin Cancer Res Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma Authors - Jialiang Wang KD Validated View Article
mouse,human	WB,IF	Free Radic Biol Med Cordycepin promotes autophagic degradation of α-synuclein via CacyBP/SIP activation for ameliorating olfactory dysfunction against Parkinson's disease Authors - Ying-Ying Gu KD Validated View Article
mouse	WB	Br J Pharmacol A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration Authors - Si-Yuan Yao View Article
Human		BMC Cancer Integrative machine learning frameworks to uncover specific protein signature in neuroendocrine cervical carcinoma Authors - Tao Shen View Article

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Validation Data Gallery

WB analysis of HeLa using 11745-1-AP

WB analysis of HEK-293 using 11745-1-AP

IP experiment of mouse brain using 11745-1-AP

IHC staining of human colon cancer using 11745-1-AP

IHC staining of human colon cancer using 11745-1-AP

IF Staining of HeLa using 11745-1-AP

FC experiment of HeLa using 11745-1-AP

CACYBP Polyclonal antibody

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET

CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.

Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma

Cordycepin promotes autophagic degradation of α-synuclein via CacyBP/SIP activation for ameliorating olfactory dysfunction against Parkinson's disease

A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration

Integrative machine learning frameworks to uncover specific protein signature in neuroendocrine cervical carcinoma

Able^™

Validation Data Gallery

WB analysis of HeLa using 11745-1-AP

WB analysis of HEK-293 using 11745-1-AP

IP experiment of mouse brain using 11745-1-AP

IHC staining of human colon cancer using 11745-1-AP

IHC staining of human colon cancer using 11745-1-AP

IF Staining of HeLa using 11745-1-AP

FC experiment of HeLa using 11745-1-AP

CACYBP Polyclonal antibody

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET

CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.

Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma

Cordycepin promotes autophagic degradation of α-synuclein via CacyBP/SIP activation for ameliorating olfactory dysfunction against Parkinson's disease

A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration

Integrative machine learning frameworks to uncover specific protein signature in neuroendocrine cervical carcinoma

Able™

Able^™