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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of rat kidney using 16961-1-AP (same clone as 16961-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of human stomach using 16961-1-AP (same clone as 16961-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of mouse ovary using 16961-1-AP (same clone as 16961-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of mouse colon using 16961-1-AP (same clone as 16961-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using CA2 Polyclonal antibody (16961-1-AP)

    WB analysis of U2OS using 16961-1-AP (same clone as 16961-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using CA2 Polyclonal antibody (16961-1-AP)

    IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

    IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

CA2 Polyclonal antibody, PBS Only

CA2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16961-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Carbonate dehydratase II, Car2, CA-II, CAII, CAC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16961-1-PBS targets CA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10680

Product name: Recombinant human CA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC011949

Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase II
Calculated molecular weight 260 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC011949
Gene Symbol CA2
Gene ID (NCBI) 760
RRIDAB_2065857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP00918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).

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