Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, rat kidney tissue, human stomach tissue, mouse ovary tissue, mouse colon tissue, mouse kidney tissue, U2OS cells |
| Positive IP detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
16961-1-AP targets CA2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10680 Product name: Recombinant human CA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC011949 Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK 相同性解析による交差性が予測される生物種 |
| Full Name | carbonic anhydrase II |
| Calculated molecular weight | 260 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC011949 |
| Gene Symbol | CA2 |
| Gene ID (NCBI) | 760 |
| RRID | AB_2065857 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CA2 antibody 16961-1-AP | Download protocol |
| WB protocol for CA2 antibody 16961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Sanhua essential oil exerts antidepressant effects in breast cancer-related depression by modulating metabolic pathways. | ||
J Genet Genomics The single cell transcriptomic landscape of recurrent giant cell tumor of bone following neoadjuvant denosumab therapy. | ||
Cell Rep Hi-C profiling in tissues reveals 3D chromatin-regulated breast tumor heterogeneity informing a looping-mediated therapeutic avenue | ||
Cell Rep Carbonic anhydrase 2 facilitates sorafenib resistance by counteracting MCT4-mediated intracellular pH dysregulation in HCC | ||
J Med Chem Intracellular Binding of Novel Fluorinated Compounds to Carbonic Anhydrase Isoforms Explored by In-Cell (19)F NMR. | ||
J Ethnopharmacol TCM compatibility-driven mechanism of Shenshuaining (SSN) capsules against renal fibrosis: Integrated metabolomic and network analyses. |