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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf78 Polyclonal antibody (14496-1-AP)

    WB analysis using 14496-1-AP (same clone as 14496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IP experiment of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human stomach cancer using 14496-1-AP (same clone as 14496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human bladder using 14496-1-AP (same clone as 14496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human bladder using 14496-1-AP (same clone as 14496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 14496-1-AP (C9orf78 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

C9orf78 Polyclonal antibody, PBS Only

C9orf78 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14496-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14496-1-PBS targets C9orf78 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5919

Product name: Recombinant human C9orf78 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-289 aa of BC007664

Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY

相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 78
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007664
Gene Symbol C9orf78
Gene ID (NCBI) 51759
RRIDAB_10644338
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ63
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.

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