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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf78 Polyclonal antibody (14496-1-AP)

    WB analysis using 14496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IP experiment of HepG2 using 14496-1-AP

    IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human stomach cancer using 14496-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bladder tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human bladder using 14496-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using C9orf78 Polyclonal antibody (14496-1-AP)

    IHC staining of human bladder using 14496-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IF Staining of HepG2 using 14496-1-AP

    Immunofluorescent analysis of HepG2 cells using 14496-1-AP (C9orf78 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C9orf78 Polyclonal antibody (14496-1-AP)

    IF Staining of HepG2 using 14496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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C9orf78 Polyclonal antibody

C9orf78 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14496-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 300 ug/ml
20 μL, 300 ug/ml
150 μL, 300 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14496-1-AP targets C9orf78 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf78 fusion protein Ag5919 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 78
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007664
Gene Symbol C9orf78
Gene ID (NCBI) 51759
RRIDAB_10644338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.

Protocols

Product Specific Protocols
WB protocol for C9orf78 antibody 14496-1-APDownload protocol
IHC protocol for C9orf78 antibody 14496-1-APDownload protocol
IF protocol for C9orf78 antibody 14496-1-APDownload protocol
IP protocol for C9orf78 antibody 14496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols