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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf117 Polyclonal antibody (21012-1-AP)

    WB analysis using 21012-1-AP (same clone as 21012-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C9orf117 Polyclonal antibody (21012-1-AP)

    IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

C9orf117 Polyclonal antibody, PBS Only

C9orf117 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21012-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21012-1-PBS targets C9orf117 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15234

Product name: Recombinant human C9orf117 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC141809

Sequence: MAPKKSVSKAGKELEVKKKGGKKEPVVAVEPPLAKEMKEFYHIQIRDLEDRLARYQRKWDELAVQEKMFRQEFEQLANNKKEIVAFLKRTLNQQVDEITDLNEQLQNLQLAKEMEKDAFEAQLAQVRHEFQETKDQLTTENIILGGKLAALEEFRLQKEEVTDKFTLLEEQVRKQENEFRDYAYNLEKKSVLDKDRLRKEIIQRVNLVANEFHKVTTNRMWETTKRAIKENNGITLQMARVSQQGMKLLQENEQLKGRQNNLCKQLELLENTQKVMARHKRGHQKIILMLTKKCQEQQQDTKEAEELRLLLSQLEQRSLQLQVDNQALKSQRDQLSLQLEQQQVDLQRLQ

相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 117
Calculated molecular weight 520 aa, 61 kDa
Observed molecular weight60 kDa
GenBank accession numberBC141809
Gene Symbol C9orf117
Gene ID (NCBI) 286207
RRIDAB_3669353
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JU67
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).

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