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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf117 Polyclonal antibody (21012-1-AP)

    WB analysis using 21012-1-AP (same clone as 21012-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C9orf117 Polyclonal antibody (21012-1-AP)

    IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

C9orf117 Polyclonal antibody, PBS Only

C9orf117 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21012-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21012-1-PBS targets C9orf117 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15234

Product name: Recombinant human C9orf117 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC141809

Sequence: MAPKKSVSKAGKELEVKKKGGKKEPVVAVEPPLAKEMKEFYHIQIRDLEDRLARYQRKWDELAVQEKMFRQEFEQLANNKKEIVAFLKRTLNQQVDEITDLNEQLQNLQLAKEMEKDAFEAQLAQVRHEFQETKDQLTTENIILGGKLAALEEFRLQKEEVTDKFTLLEEQVRKQENEFRDYAYNLEKKSVLDKDRLRKEIIQRVNLVANEFHKVTTNRMWETTKRAIKENNGITLQMARVSQQGMKLLQENEQLKGRQNNLCKQLELLENTQKVMARHKRGHQKIILMLTKKCQEQQQDTKEAEELRLLLSQLEQRSLQLQVDNQALKSQRDQLSLQLEQQQVDLQRLQ

相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 117
Calculated molecular weight 520 aa, 61 kDa
Observed molecular weight60 kDa
GenBank accession numberBC141809
Gene Symbol C9orf117
Gene ID (NCBI) 286207
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JU67
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).

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