Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, Raji cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21012-1-AP targets C9orf117 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C9orf117 fusion protein Ag15234 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 9 open reading frame 117 |
| Calculated molecular weight | 520 aa, 61 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC141809 |
| Gene Symbol | C9orf117 |
| Gene ID (NCBI) | 286207 |
| RRID | AB_3669353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JU67 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf117 antibody 21012-1-AP | Download protocol |
| IHC protocol for C9orf117 antibody 21012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |