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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1r Recombinant antibody (85029-4-RR)

    WB analysis of human plasma using 85029-4-RR (same clone as 85029-4-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 85029-4-RR (C1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1r Recombinant antibody (85029-4-RR)

    IHC staining of mouse liver using 85029-4-RR (same clone as 85029-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1r Recombinant antibody (85029-4-RR)

    IHC staining of mouse liver using 85029-4-RR (same clone as 85029-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85029-4-RR

    Affinity and Kinetic Characterization of 85029-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85029-4-RR against Human C1r were performed. The affinity constant is below 1 pM.

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C1r Recombinant antibody, PBS Only

C1r Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85029-4-PBS
Clone No.242596F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.4.21.41, Complement C1r subcomponent light chain, Complement C1r subcomponent heavy chain, Complement C1r subcomponent, Complement C1r

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85029-4-PBS targets C1r in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1r fusion protein Ag11390 相同性解析による交差性が予測される生物種
Full Name complement component 1, r subcomponent
Calculated molecular weight 705 aa, 80 kDa
Observed molecular weight85 kDa
GenBank accession numberBC035220
Gene Symbol C1R
Gene ID (NCBI) 715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00736
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation.