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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1r Recombinant antibody (85029-4-RR)

    WB analysis of human plasma using 85029-4-RR

    human plasma was subjected to SDS PAGE followed by western blot with 85029-4-RR (C1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1r Recombinant antibody (85029-4-RR)

    IHC staining of mouse liver using 85029-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1r Recombinant antibody (85029-4-RR)

    IHC staining of mouse liver using 85029-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85029-4-RR

    Affinity and Kinetic Characterization of 85029-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85029-4-RR against Human C1r were performed. The affinity constant is below 1 pM.

C1r Recombinant monoclonal antibody

C1r Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85029-4-RR
Clone No.242596F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

242596F5, Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85029-4-RR targets C1r in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11390

Product name: Recombinant human C1R protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 356-705 aa of BC035220

Sequence: AVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSRESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGALLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIALLELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRMDVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKEMEEED

相同性解析による交差性が予測される生物種
Full Name complement component 1, r subcomponent
Calculated molecular weight 705 aa, 80 kDa
Observed molecular weight85 kDa
GenBank accession numberBC035220
Gene Symbol C1R
Gene ID (NCBI) 715
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP00736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation.

Protocols

Product Specific Protocols
IHC protocol for C1r antibody 85029-4-RRDownload protocol
WB protocol for C1r antibody 85029-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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