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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated C1QBP Recombinant antibody

C1QBP Recombinant Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3K20

Cat no : CL488-80490

Synonyms

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-80490

    IF Staining of HeLa using CL488-80490

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 C1QBP antibody (CL488-80490, Clone: 3K20 ) at dilution of 1:100, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • FC experiment of HeLa using CL488-80490

    FC experiment of HeLa using CL488-80490

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human C1QBP (CL488-80490, Clone:3K20) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80490-1-PBS

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-80490 targets C1QBP in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1QBP fusion protein Ag19773 相同性解析による交差性が予測される生物種
Full Name complement component 1, q subcomponent binding protein
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight32 kDa
GenBank accession numberBC013731
Gene symbol C1QBP
Gene ID (NCBI) 708
RRIDAB_2919578
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 C1QBP antibody CL488-80490Download protocol
FC protocol for CL Plus 488 C1QBP antibody CL488-80490Download protocol
Standard Protocols
Click here to view our Standard Protocols