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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of A431 using 24553-1-AP (same clone as 24553-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of COLO 320 using 24553-1-AP (same clone as 24553-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of HepG2 using 24553-1-AP (same clone as 24553-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human tonsillitis using 24553-1-AP (same clone as 24553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human tonsillitis using 24553-1-AP (same clone as 24553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human spleen using 24553-1-AP (same clone as 24553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human spleen using 24553-1-AP (same clone as 24553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

C15orf53 Polyclonal antibody, PBS Only

C15orf53 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24553-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24553-1-PBS targets C15orf53 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18630

Product name: Recombinant human C15orf53 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-179 aa of BC119016

Sequence: MELQGAQEDLGISLSSPRRNHETRPGSKAKGRSSICLQASVWMAGGKLRLRASEHLTQGHQQELRDWNLGEDASLLFSKSPFGAGKLIQAPAHVFRQCWVQGNAWISCITKFDSKRSPEVASSPSYLTVPRRSPLPVFLRPSDRCVCGGCYLGKSTRRRACQSLLSDPLGVTFPTQTRP

相同性解析による交差性が予測される生物種
Full Name chromosome 15 open reading frame 53
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight ~30 kDa
GenBank accession numberBC119016
Gene Symbol C15orf53
Gene ID (NCBI) 400359
RRIDAB_2879605
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NAA6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C15orf53 has been identified on chromosome 15q14. It is probably neither causative for the etiology of BD (Bipolar disorder) nor for SCZD10 (periodic catatonia). Gene expression analysis revealed that C15orf53 was expressed in a subpopulation of leukocytes, but not in human post-mortem limbic brain tissue. In our detection this antibody always detect ~30 kDa band even the amino acids is 20 kDa.

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