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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of A431 using 24553-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of COLO 320 using 24553-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C15orf53 Polyclonal antibody (24553-1-AP)

    WB analysis of HepG2 using 24553-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human tonsillitis using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human tonsillitis using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human spleen using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using C15orf53 Polyclonal antibody (24553-1-AP)

    IHC staining of human spleen using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens).

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C15orf53 Polyclonal antibody

C15orf53 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24553-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C15orf53

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24553-1-AP targets C15orf53 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C15orf53 fusion protein Ag18630 相同性解析による交差性が予測される生物種
Full Name chromosome 15 open reading frame 53
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight ~30 kDa
GenBank accession numberBC119016
Gene Symbol C15orf53
Gene ID (NCBI) 400359
RRIDAB_2879605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NAA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C15orf53 has been identified on chromosome 15q14. It is probably neither causative for the etiology of BD (Bipolar disorder) nor for SCZD10 (periodic catatonia). Gene expression analysis revealed that C15orf53 was expressed in a subpopulation of leukocytes, but not in human post-mortem limbic brain tissue. In our detection this antibody always detect ~30 kDa band even the amino acids is 20 kDa.

Protocols

Product Specific Protocols
WB protocol for C15orf53 antibody 24553-1-APDownload protocol
IHC protocol for C15orf53 antibody 24553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols