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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C14orf166 Polyclonal antibody (19848-1-AP)

    WB analysis using 19848-1-AP (same clone as 19848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using C14orf166 Polyclonal antibody (19848-1-AP)

    WB analysis of A549 using 19848-1-AP (same clone as 19848-1-PBS)

    WB result of C14orf166 antibody (19848-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C14orf166 transfected A549 cells. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    WB analysis of mouse pancreas using 19848-1-AP (same clone as 19848-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    WB analysis of mouse lung using 19848-1-AP (same clone as 19848-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using C14orf166 Polyclonal antibody (19848-1-AP)

    IP experiment of Jurkat using 19848-1-AP (same clone as 19848-1-PBS)

    IP result of anti-C14orf166 (IP:19848-1-AP, 3ug; Detection:19848-1-AP 1:700) with Jurkat cells lysate 1080ug. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C14orf166 Polyclonal antibody (19848-1-AP)

    IHC staining of human prostate cancer using 19848-1-AP (same clone as 19848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using C14orf166 Polyclonal antibody (19848-1-AP)

    IF Staining of Jurkat using 19848-1-AP (same clone as 19848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using C14orf166 antibody (19848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

C14orf166 Polyclonal antibody, PBS Only

C14orf166 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19848-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19848-1-PBS targets C14orf166 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13900

Product name: Recombinant human CLE; C14orf166 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC001722

Sequence: MFRRKLTALDYHNPAGFNCKDETEFRNFIVWLEDQKIRHYKIEDRGNLRNIHSSDWPKFFEKYLRDVNCPFKIQDRQEAIDWLLGLAVRLEYGDNAEKYKDLVPDNSKTADNATKNAEPLINLDVNNPDFKAGVMALANLLQIQRHDDYLVMLKAIRILVQERLTQDAVAKANQTKEGLPVALDKHILGFDTGDAVLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR

相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 166
Calculated molecular weight 244 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC001722
Gene Symbol C14orf166
Gene ID (NCBI) 51637
RRIDAB_10646485
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y224
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.

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