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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    WB analysis of human skeletal muscle using 21183-1-AP (same clone as 21183-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using C13orf18 Polyclonal antibody (21183-1-AP)

    WB analysis of MCF-7 using 21183-1-AP (same clone as 21183-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21183-1-AP (C13orf18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21183-1-AP (C13orf18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human colon using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human colon using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP (same clone as 21183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using C13orf18 Polyclonal antibody (21183-1-AP)

    IF Staining of U-251 using 21183-1-AP (same clone as 21183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C13orf18 antibody (21183-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21183-1-PBS in a different storage buffer formulation.

C13orf18 Polyclonal antibody, PBS Only

C13orf18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21183-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Pacer, Protein associated with UVRAG as autophagy enhancer, Protein Rubicon-like, RUBCNL

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21183-1-PBS targets C13orf18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15474

Product name: Recombinant human C13orf18 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-306 aa of BC032311

Sequence: MVSQSTVRQDSPVEPWEGISDHSGIIDGSPRLLNTDHPPCQLDIRLMRHKAVWINPQDVQQQPQDLQSQVPAAGNSGTHFVTDAASPSGPSPSCLGDSLAETTLSEDTTDSVGSASPHGSSEKSSSFSLSSTEVHMVRPGYSHRVSLPTSPGILATSPYPETDSAFFEPSHLTSAADEGAVQVSRRTISSNSFSPEVFVLPVDVEKENAHFYVADMIISAMEKMKCNILSQQQTESWSKEVSGLLGSDQPDSEMTFDTNIKQESGSSTSSYSGYEGCAVLQVSPVTETRTYHDVKEICKCDVDEFV

相同性解析による交差性が予測される生物種
Full Name chromosome 13 open reading frame 18
Calculated molecular weight 662 aa, 73 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC032311
Gene Symbol C13orf18
Gene ID (NCBI) 80183
RRIDAB_10733098
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H714
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C13orf18 (also known as Pacer), now formally designated RUBCNL (Rubicon-like autophagy enhancer), is a protein containing an EF-hand calcium-binding domain with potential transporter functions. Initially identified as a candidate tumor suppressor gene, it is frequently silenced in cervical cancer due to promoter hypermethylation. Its re-expression inhibits cervical cancer cell proliferation, suggesting tumor-suppressing activity. Furthermore, research has revealed that polymorphisms in the 3'UTR region of the C13orf18 gene are associated with amyotrophic lateral sclerosis (ALS), suggesting its potential involvement in autophagy regulation and the pathogenesis of neurodegenerative diseases.

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