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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    WB analysis of human skeletal muscle using 21183-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using C13orf18 Polyclonal antibody (21183-1-AP)

    WB analysis of MCF-7 using 21183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human colon using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using C13orf18 Polyclonal antibody (21183-1-AP)

    IHC staining of human colon using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using C13orf18 Polyclonal antibody (21183-1-AP)

    IF Staining of U-251 using 21183-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C13orf18 antibody (21183-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

C13orf18 Polyclonal antibody

C13orf18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21183-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Pacer, Protein associated with UVRAG as autophagy enhancer, Protein Rubicon-like, RUBCNL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21183-1-AP targets C13orf18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15474

Product name: Recombinant human C13orf18 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-306 aa of BC032311

Sequence: MVSQSTVRQDSPVEPWEGISDHSGIIDGSPRLLNTDHPPCQLDIRLMRHKAVWINPQDVQQQPQDLQSQVPAAGNSGTHFVTDAASPSGPSPSCLGDSLAETTLSEDTTDSVGSASPHGSSEKSSSFSLSSTEVHMVRPGYSHRVSLPTSPGILATSPYPETDSAFFEPSHLTSAADEGAVQVSRRTISSNSFSPEVFVLPVDVEKENAHFYVADMIISAMEKMKCNILSQQQTESWSKEVSGLLGSDQPDSEMTFDTNIKQESGSSTSSYSGYEGCAVLQVSPVTETRTYHDVKEICKCDVDEFV

相同性解析による交差性が予測される生物種
Full Name chromosome 13 open reading frame 18
Calculated molecular weight 662 aa, 73 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC032311
Gene Symbol C13orf18
Gene ID (NCBI) 80183
RRIDAB_10733098
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H714
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for C13orf18 antibody 21183-1-APDownload protocol
IHC protocol for C13orf18 antibody 21183-1-APDownload protocol
WB protocol for C13orf18 antibody 21183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Architecture of the human interactome defines protein communities and disease networks.

Authors - Edward L Huttlin
View Article
humanWB

Precis Clin Med

Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis

Authors - Fengqin Zhu
View Article
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