Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Transfected CHO cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98867-1-RR targets C-peptide in IHC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | INS |
| Calculated molecular weight | 110aa, 12 kDa |
| GenBank accession number | NM_000207 |
| Gene Symbol | INS |
| Gene ID (NCBI) | 3630 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01308 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
C-peptide (connecting peptide) is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. It is cleaved from proinsulin in the pancreatic beta cells, and one molecule of C-peptide is released for every molecule of insulin (PMID: 6759081). C-peptide is secreted at a relatively steady rate over long duration, making it an effective biomarker for endogenous insulin secretion (PMID: 41694179).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for C-peptide antibody 98867-1-RR | Download protocol |
| IHC protocol for C-peptide antibody 98867-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |