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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pancreas tissue using Anti-Human C-peptide Rabbit Recombinant Antibody (98867-1-RR)

    IHC staining of human pancreas using 98867-1-RR (same clone as 98867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Anti-Human C-peptide Rabbit Recombinant Antibody (98867-1-RR)

    IHC staining of human pancreas using 98867-1-RR (same clone as 98867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected CHO using Anti-Human C-peptide Rabbit Recombinant Antibody (98867-1-RR)

    FC experiment of Transfected CHO using 98867-1-RR (same clone as 98867-1-PBS)

    1x10^6 CHO cells or C-Peptide transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human C-Peptide Rabbit RecAb (98867-1-RR, Clone: 253514D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98867-1-PBS in a different storage buffer formulation.

Anti-Human C-peptide Rabbit Recombinant Antibody, PBS Only

C-peptide Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98867-1-PBS
Clone No.253514D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra)

Synonyms

C peptide, INS C-Peptide, Insulin C-Peptide

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98867-1-PBS targets C-peptide in IHC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name INS
Calculated molecular weight110aa, 12 kDa
GenBank accession numberNM_000207
Gene Symbol INS
Gene ID (NCBI) 3630
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01308
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C-peptide (connecting peptide) is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. It is cleaved from proinsulin in the pancreatic beta cells, and one molecule of C-peptide is released for every molecule of insulin (PMID: 6759081). C-peptide is secreted at a relatively steady rate over long duration, making it an effective biomarker for endogenous insulin secretion (PMID: 41694179).

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