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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Tubulin Polyclonal antibody (10063-2-AP)

    WB analysis using 10063-2-AP (same clone as 10063-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10063-2-AP (Beta Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10063-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Beta Tubulin Polyclonal antibody (10063-2-AP)

    IHC staining of human gliomas using 10063-2-AP (same clone as 10063-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10063-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Beta Tubulin Polyclonal antibody (10063-2-AP)

    IHC staining of human gliomas using 10063-2-AP (same clone as 10063-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10063-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Beta Tubulin Polyclonal antibody (10063-2-AP)

    IHC staining of mouse cerebellum using 10063-2-AP (same clone as 10063-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10063-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Beta Tubulin Polyclonal antibody (10063-2-AP)

    IHC staining of mouse cerebellum using 10063-2-AP (same clone as 10063-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10063-2-PBS in a different storage buffer formulation.

Beta Tubulin Polyclonal antibody, PBS Only

Beta Tubulin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10063-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Tubulin beta-2B chain, TUBB2B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10063-2-PBS targets Beta Tubulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0108

Product name: Recombinant human tubulin-beta protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 11-266 aa of BC001352

Sequence: QCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF

相同性解析による交差性が予測される生物種
Full Name tubulin, beta 2B
Calculated molecular weight 50 kDa
Observed molecular weight50kda
GenBank accession numberBC001352
Gene Symbol Beta Tubulin
Gene ID (NCBI) 347733
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVA1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

There are five tubulins in human cells. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.

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