Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10063-2-AP targets Beta Tubulin in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0108 Product name: Recombinant human tubulin-beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-266 aa of BC001352 Sequence: QCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF 相同性解析による交差性が予測される生物種 |
| Full Name | tubulin, beta 2B |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50kda |
| GenBank accession number | BC001352 |
| Gene Symbol | Beta Tubulin |
| Gene ID (NCBI) | 347733 |
| RRID | AB_2210536 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
There are five tubulins in human cells. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Beta Tubulin antibody 10063-2-AP | Download protocol |
| WB protocol for Beta Tubulin antibody 10063-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biol Psychiatry Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins. | ||
J Am Soc Nephrol Tubular von Hippel-Lindau knockout protects against rhabdomyolysis-induced AKI. | ||
Nucleic Acids Res Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum. | ||
Ecotoxicol Environ Saf Cinchophen induces RPA1 related DNA damage and apoptosis to impair ENS development of zebrafish | ||
Cancer Biol Med IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation. |