CoraLite® Plus 488-conjugated Beta Galactosidase Monoclonal antibody
Beta Galactosidase Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4F4F4
Cat no : CL488-66586
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66586 targets Beta Galactosidase in IF applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Beta Galactosidase fusion protein Ag8069 相同性解析による交差性が予測される生物種 |
| Full Name | galactosidase, beta 1 |
| Calculated molecular weight | 76 kDa |
| Observed molecular weight | 64-66 kDa, 76-85 kDa |
| GenBank accession number | BC007493 |
| Gene symbol | GLB1 |
| Gene ID (NCBI) | 2720 |
| RRID | AB_2919348 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Beta Galactosidase antibody CL488-66586 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
