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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated Beta Catenin Polyclonal an (CL594-17565)

    IF Staining of MCF-7 using CL594-17565

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 Beta Catenin antibody (CL594-17565) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using CoraLite®594-conjugated Beta Catenin Polyclonal an (CL594-17565)

    IF Staining of T-47D using CL594-17565

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594 Beta Catenin antibody (CL594-17565) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated Beta Catenin Polyclonal an (CL594-17565)

    IF Staining of MCF-7 using CL594-17565

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 Beta Catenin antibody (CL594-17565) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated Beta Catenin Polyclonal an (CL594-17565)

    FC experiment of MCF-7 using CL594-17565

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Beta Catenin (CL594-17565) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-17565

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells, T-47D cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17565 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11763

Product name: Recombinant human B-catenin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 433-781 aa of BC058926

Sequence: KNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL

相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2919845
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
IF protocol for CL594 Beta Catenin antibody CL594-17565Download protocol
Standard Protocols
Click here to view our Standard Protocols
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