Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83683-8 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2284 Product name: Recombinant Human B2M protein (mFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-mFc Tag: C-mFc Domain: 21-119 aa of BC032589 Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 相同性解析による交差性が予測される生物種 |
| Full Name | beta-2-microglobulin |
| Calculated molecular weight | 119 aa, 14 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC032589 |
| Gene Symbol | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61769 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Beta-2-Microglobulin antibody CL488-83683-8 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |