Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, U-251 cells, Raji cells, K-562 cells, rat heart tissue, fetal human brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85336-1-RR targets BUD31 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2373 Product name: Recombinant human BUD31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC022821 Sequence: MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 相同性解析による交差性が予測される生物種 |
| Full Name | BUD31 homolog (S. cerevisiae) |
| Calculated molecular weight | 144 aa, 17 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC022821 |
| Gene Symbol | BUD31 |
| Gene ID (NCBI) | 8896 |
| RRID | AB_3744145 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P41223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BUD31 antibody 85336-1-RR | Download protocol |
| WB protocol for BUD31 antibody 85336-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |