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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUD31 Recombinant antibody (85336-1-RR)

    WB analysis using 85336-1-RR (same clone as 85336-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85336-1-RR (BUD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BUD31 Recombinant antibody (85336-1-RR)

    WB analysis using 85336-1-RR (same clone as 85336-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85336-1-RR (BUD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85336-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BUD31 Recombinant antibody (85336-1-RR)

    IHC staining of mouse brain using 85336-1-RR (same clone as 85336-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85336-1-RR (BUD31 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85336-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BUD31 Recombinant antibody (85336-1-RR)

    IHC staining of mouse brain using 85336-1-RR (same clone as 85336-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85336-1-RR (BUD31 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85336-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85336-1-RR

    Affinity and Kinetic Characterization of 85336-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85336-1-RR against Human BUD31 were performed. The affinity constant is below 1 pM.

BUD31 Recombinant monoclonal antibody, PBS Only

BUD31 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85336-1-PBS
Clone No.242551D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

242551D3, EDG 2, EDG2, fSAP17, G10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85336-1-PBS targets BUD31 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2373

Product name: Recombinant human BUD31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-144 aa of BC022821

Sequence: MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG

相同性解析による交差性が予測される生物種
Full Name BUD31 homolog (S. cerevisiae)
Calculated molecular weight 144 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC022821
Gene Symbol BUD31
Gene ID (NCBI) 8896
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP41223
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.