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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of MCF-7 using 16482-1-AP (same clone as 16482-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of human testis using 16482-1-AP (same clone as 16482-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    IF Staining of HepG2 using 16482-1-AP (same clone as 16482-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

BTN2A2 Polyclonal antibody, PBS Only

BTN2A2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16482-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16482-1-PBS targets BTN2A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9603

Product name: Recombinant human BTN2A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-523 aa of BC014021

Sequence: VSICCIKKLQREKKILSGEKKVEQEEKEIAQQLQEELRWRRTFLHAADVVLDPDTAHPELFLSEDRRSVRRGPYRQRVPDNPERFDSQPCVLGWESFASGKHYWEVEVENVMVWTVGVCRHSVERKGEVLLIPQNGFWTLEMFGNQYRALSSPERILPLKESLCRVGVFLDYEAGDVSFYNMRDRSHIYTCPRSAFTVPVRPFFRLGSDDSPIFICPALTGASGVMVPEEGLKLHRVGTHQSL

相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A2
Calculated molecular weight 523 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC014021
Gene Symbol BTN2A2
Gene ID (NCBI) 10385
RRIDAB_2228110
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVV5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

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