• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of MCF-7 using 16482-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of human testis using 16482-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    IF Staining of HepG2 using 16482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

BTN2A2 Polyclonal antibody

BTN2A2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16482-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

BTF2, BT2.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, NIH/3T3 cells, MCF-7 cells, human testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16482-1-AP targets BTN2A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9603

Product name: Recombinant human BTN2A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-523 aa of BC014021

Sequence: VSICCIKKLQREKKILSGEKKVEQEEKEIAQQLQEELRWRRTFLHAADVVLDPDTAHPELFLSEDRRSVRRGPYRQRVPDNPERFDSQPCVLGWESFASGKHYWEVEVENVMVWTVGVCRHSVERKGEVLLIPQNGFWTLEMFGNQYRALSSPERILPLKESLCRVGVFLDYEAGDVSFYNMRDRSHIYTCPRSAFTVPVRPFFRLGSDDSPIFICPALTGASGVMVPEEGLKLHRVGTHQSL

相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A2
Calculated molecular weight 523 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC014021
Gene Symbol BTN2A2
Gene ID (NCBI) 10385
RRIDAB_2228110
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVV5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

Protocols

Product Specific Protocols
WB protocol for BTN2A2 antibody 16482-1-APDownload protocol
IF protocol for BTN2A2 antibody 16482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols