BSA depletion from various antibodies. Proteintech BSA Depletion Agarose was validated with antibodies from various suppliers and with varying BSA content (from 0 – 0.1%). IgGs run at ~150 kDa (non-reducing SDS PAGE), BSA runs at ~50 kDa. Comparing the Input (In) vs. output (Out) lanes consistently shows successful removal of BSA with minimal residual BSA in the output fraction. Poly = Polyclonal, Ms = Mouse, Rb = Rabbit, Ch, Sp = Sheep, Ch = Chicken, Rt = Rat

BSA depletion benchmarking.
Proteintech BSA Depletion Beads (Lane 4) were evaluated for BSA depletion efficiency and compared with competitor products. The BSA reference (Lane 2) contains 0.1% BSA and is contaminated with bovine IgG (which is normal). The input antibody contains 1% BSA (lane 3). Proteintech BSA Depletion Beads removes approximately 90% of the BSA. In contrast, competitor products leave high residual BSA levels, thus also failing to remove bovine IgG that interferes with accurate quantification of the target IgG (Lanes 5 and 6). Other competitor products also exhibit reduced recovery of the target IgG (Lane 7), most evident from the Western blot result. Western blot analysis using an anti-rabbit IgG antibody confirms efficient recovery of the target IgG following Proteintech BSA Depletion Beads treatment, with no evident loss of the target IgG (here: rabbit IgG).

Immunoprecipitation of serum albumin from different species using BSA Depletion Agarose. BSA Depletion beads were used to test for cross reactivity to albumnin from other species. As expected, strong cross-reactivity to albumin from closely related species (bovid family: sheep, goat) can be observed. Other serum albumin species do not bind to the beads (e.g., HSA from humans)

BSA depletion benchmarking.
Proteintech BSA Depletion Agarose (Lane 3) was evaluated for BSA depletion efficiency and compared with competitor products. The starting antibody (Lane 2) contains 0.01% BSA. Proteintech BSA Depletion Beads remove approximately 95% of the BSA. In contrast, competitor products partly leave higher residual BSA levels. Other competitor products also exhibit reduced recovery of the target IgG (Lane 6).

Reference table for bead usage per depletion.
Table 1 shows the recommended bead volume for each antibody solution volume (top row) and corresponding BSA concentration (%) (first column).
Table 2 shows the expected number of depletions for each unit size (1 mL, 2 mL, and 5 mL), based on the antibody solution volume and BSA concentration, using the recommended bead volumes specified in Table 1.