Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-30186 targets BRPF1 in FC (Intra) applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32828 Product name: Recombinant human BRPF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 741-880 aa of BC053851 Sequence: QAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGL 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain and PHD finger containing, 1 |
| Calculated molecular weight | 1220 aa, 138 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC053851 |
| Gene Symbol | BRPF1 |
| Gene ID (NCBI) | 7862 |
| RRID | AB_3672844 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55201 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 BRPF1 antibody CL488-30186 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |