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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BNIPL Polyclonal antibody (13262-1-AP)

    WB analysis using 13262-1-AP (same clone as 13262-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BNIPL Polyclonal antibody (13262-1-AP)

    WB analysis using 13262-1-AP (same clone as 13262-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using BNIPL Polyclonal antibody (13262-1-AP)

    IHC staining of mouse skin using 13262-1-AP (same clone as 13262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using BNIPL Polyclonal antibody (13262-1-AP)

    IHC staining of mouse skin using 13262-1-AP (same clone as 13262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13262-1-PBS in a different storage buffer formulation.

BNIPL Polyclonal antibody, PBS Only

BNIPL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13262-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein, BNIP S, BNIPL 1, BNIPL 2, BNIPL1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13262-1-PBS targets BNIPL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3921

Product name: Recombinant human BNIPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-357 aa of BC027868

Sequence: MGTIQEAGKKTDVGVREIAEAPELGAALRHGELELKEEWQDEEFPRLLPEEAGTSEDPEDPKGDSQAAAGTPSTLALCGQRPMRKRLSAPELRLSLTKGPGNDGASPTQSAPSSPDGSSDLEIDELETPSDSEQLDSGHEFEWEDELPRAEGLGTSETAERLGRGCMWDVTGEDGHHWRVFRMGPREQRVDMTVIEPYKKVLSHGGYHGDGLNAVILFASCYLPRSSIPNYTYVMEHLFRYMVGTLELLVAENYLLVHLSGGTSRAQVPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLALLRPFISSKFTRKIRFLDSLGELAQLISLDQVHIPEAVRQLDRDLHGSGGT

相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kD interacting protein like
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight43 kDa
GenBank accession numberBC027868
Gene Symbol BNIPL
Gene ID (NCBI) 149428
RRIDAB_3085410
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z465
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.

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