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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BNIPL Polyclonal antibody (13262-1-AP)

    WB analysis using 13262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BNIPL Polyclonal antibody (13262-1-AP)

    WB analysis using 13262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BNIPL Polyclonal antibody (13262-1-AP)

    IHC staining of human lung cancer using 13262-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13262-1-AP (BNIPL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BNIPL Polyclonal antibody (13262-1-AP)

    IHC staining of human colon cancer using 13262-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13262-1-AP (BNIPL Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BNIPL Polyclonal antibody (13262-1-AP)

    IHC staining of human colon cancer using 13262-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13262-1-AP (BNIPL Antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

BNIPL Polyclonal antibody

BNIPL Polyclonal Antibody for WB, ELISA
Cat No. 13262-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BNIP S, BNIPL, BNIPL 1, BNIPL 2, BNIPL1, BNIPL2, PP753

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue, rat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13262-1-AP targets BNIPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNIPL fusion protein Ag3921 相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kD interacting protein like
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight43 kDa
GenBank accession numberBC027868
Gene Symbol BNIPL
Gene ID (NCBI) 149428
RRIDAB_3085410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z465
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.

Protocols

Product Specific Protocols
WB protocol for BNIPL antibody 13262-1-APDownload protocol
IHC protocol for BNIPL antibody 13262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols