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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    WB analysis of HT-1080 using 66161-1-Ig (same clone as 66161-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using BMI1 Monoclonal antibody (66161-1-Ig)

    WB analysis of U2OS using 66161-1-Ig (same clone as 66161-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    WB analysis of U-937 using 66161-1-Ig (same clone as 66161-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IP result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BMI1 Monoclonal antibody (66161-1-Ig)

    IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BMI1 Monoclonal antibody (66161-1-Ig)

    IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using BMI1 Monoclonal antibody (66161-1-Ig)

    IHC staining of human colon cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BMI1 Monoclonal antibody (66161-1-Ig)

    IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    IF Staining of COS-7 using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunofluorescent analysis of COS-7 cells using 66161-1-Ig (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BMI1 Monoclonal antibody (66161-1-Ig)

    IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BMI1 antibody (66161-1-Ig, Clone: 2E10A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51443-1

    Cytometric bead array sample test of MP51443-1

    Cytometric bead array sample test of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS.

  • Cytometric bead array standard curve of MP51443-1

    Cytometric bead array standard curve of MP51443-1

    Cytometric bead array standard curve of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS. Standard:Ag21284. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO Validated

BMI1 Monoclonal antibody, PBS Only (Detector)

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 66161-1-PBS
Clone No.2E10A11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

2E10A11, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66161-1-PBS targets BMI1 as part of a matched antibody pair:

MP51443-1: 66161-2-PBS capture and 66161-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, monkey
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21284

Product name: Recombinant human BMI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-326 aa of BC011652

Sequence: MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

相同性解析による交差性が予測される生物種
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight37-43 kDa
GenBank accession numberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
RRIDAB_2881557
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35226
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.