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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30254-1-PBS in a different storage buffer formulation.

BLM Polyclonal antibody, PBS Only

BLM Polyclonal Antibody for WB, Indirect ELISA
Cat No. 30254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

RECQL3, RECQL2, RECQ2, BS

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30254-1-PBS targets BLM in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32996

Product name: Recombinant human BLM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1235-1417 aa of

Sequence: VHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

相同性解析による交差性が予測される生物種
Full Name Bloom syndrome, RecQ helicase-like
Calculated molecular weight159 kDa
Observed molecular weight160-175 kDa
Gene Symbol BLM
Gene ID (NCBI) 641
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54132
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.

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