Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HEK-293T cells, HeLa cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30254-1-AP targets BLM in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32996 Product name: Recombinant human BLM protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: VHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS 相同性解析による交差性が予測される生物種 |
| Full Name | Bloom syndrome, RecQ helicase-like |
| Calculated molecular weight | 159 kDa |
| Observed molecular weight | 160-175 kDa |
| Gene Symbol | BLM |
| Gene ID (NCBI) | 641 |
| RRID | AB_3086282 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54132 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BLM antibody 30254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |