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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BHMT Recombinant antibody (86372-1-RR)

    WB analysis using 86372-1-RR (same clone as 86372-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86372-1-RR (BHMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat liver tissue using BHMT Recombinant antibody (86372-1-RR)

    IP experiment of rat liver using 86372-1-RR (same clone as 86372-1-PBS)

    IP result of anti-BHMT (IP:86372-1-RR, 4ug; Detection:86372-1-RR 1:3000) with rat liver tissue lysate 3080 ug. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86372-1-RR

    Affinity and Kinetic Characterization of 86372-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86372-1-RR against Human BHMT were performed. The affinity constant is below 1 pM.

BHMT Recombinant monoclonal antibody, PBS Only

BHMT Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 86372-1-PBS
Clone No.250978E3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86372-1-PBS targets BHMT in WB, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8738

Product name: Recombinant human BHMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-406 aa of BC012616

Sequence: MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ

相同性解析による交差性が予測される生物種
Full Name betaine-homocysteine methyltransferase
Calculated molecular weight 406 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC012616
Gene Symbol BHMT
Gene ID (NCBI) 635
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ93088
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that Involved in the regulation of homocysteine metabolism. BHMT catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline(PMID: 8798461, 10529246).