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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis using 23101-1-AP (same clone as 23101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HEK-293 using 23101-1-AP (same clone as 23101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HeLa using 23101-1-AP (same clone as 23101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

BEND3 Polyclonal antibody, PBS Only

BEND3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23101-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23101-1-PBS targets BEND3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19137

Product name: Recombinant human BEND3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 493-828 aa of BC131628

Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

相同性解析による交差性が予測される生物種
Full Name BEN domain containing 3
Calculated molecular weight 828 aa, 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5T5X7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

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