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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis using 23101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis of HEK-293 using 23101-1-AP

    WB result of BEND3 antibody (23101-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-BEND3 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HEK-293 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HeLa using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of A431 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BEND3 antibody (23101-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • KD/KO Validated

BEND3 Polyclonal antibody

BEND3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23101-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NCCIT cells, U2OS cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23101-1-AP targets BEND3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19137

Product name: Recombinant human BEND3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 493-828 aa of BC131628

Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

相同性解析による交差性が予測される生物種
Full Name BEN domain containing 3
Calculated molecular weight 828 aa, 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5T5X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

Protocols

Product Specific Protocols
IF protocol for BEND3 antibody 23101-1-APDownload protocol
WB protocol for BEND3 antibody 23101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma

Authors - Zhao Ding
View Article
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