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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated BCR Monoclonal antibody

BCR Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C2E3

Cat no : CL488-67260

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-67260

    FC experiment of K-562 using CL488-67260

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCR (CL488-67260, Clone:2C2E3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67260-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67260 targets BCR in FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCR fusion protein Ag28953 相同性解析による交差性が予測される生物種
Full Name breakpoint cluster region
Calculated molecular weight 143 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC066122
Gene symbol BCR
Gene ID (NCBI) 613
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 BCR antibody CL488-67260Download protocol
Standard Protocols
Click here to view our Standard Protocols