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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCORL1 Polyclonal antibody (24315-1-AP)

    WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BCORL1 Polyclonal antibody (24315-1-AP)

    WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse colon using 24315-1-AP (same clone as 24315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using BCORL1 Polyclonal antibody (24315-1-AP)

    IF Staining of U2OS using 24315-1-AP (same clone as 24315-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

BCORL1 Polyclonal antibody, PBS Only

BCORL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24315-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24315-1-PBS targets BCORL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19522

Product name: Recombinant human BCORL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC156742

Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA

相同性解析による交差性が予測される生物種
Full Name BCL6 co-repressor-like 1
Calculated molecular weight 1711 aa, 183 kDa
Observed molecular weight200 kDa
GenBank accession numberBC156742
Gene Symbol BCORL1
Gene ID (NCBI) 63035
RRIDAB_3669432
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5H9F3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.

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