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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCORL1 Polyclonal antibody (24315-1-AP)

    WB analysis using 24315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BCORL1 Polyclonal antibody (24315-1-AP)

    WB analysis using 24315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse colon using 24315-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse testis using 24315-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BCORL1 Polyclonal antibody (24315-1-AP)

    IHC staining of mouse testis using 24315-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using BCORL1 Polyclonal antibody (24315-1-AP)

    IF Staining of U2OS using 24315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BCORL1 Polyclonal antibody

BCORL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CXorf10

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HuH-7 cells
Positive IHC detected inmouse testis tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24315-1-AP targets BCORL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19522

Product name: Recombinant human BCORL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC156742

Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA

相同性解析による交差性が予測される生物種
Full Name BCL6 co-repressor-like 1
Calculated molecular weight 1711 aa, 183 kDa
Observed molecular weight200 kDa
GenBank accession numberBC156742
Gene Symbol BCORL1
Gene ID (NCBI) 63035
RRIDAB_3669432
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5H9F3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.

Protocols

Product Specific Protocols
IF protocol for BCORL1 antibody 24315-1-APDownload protocol
IHC protocol for BCORL1 antibody 24315-1-APDownload protocol
WB protocol for BCORL1 antibody 24315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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