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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Recombinant antibody (84882-5-RR)

    IHC staining of mouse spleen using 84882-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Recombinant antibody (84882-5-RR)

    IHC staining of mouse spleen using 84882-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCL3 Recombinant antibody (84882-5-RR)

    IF Staining of HeLa using 84882-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCL3 Recombinant antibody (84882-5-RR)

    IF Staining of HepG2 using 84882-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84882-5-RR

    Affinity and Kinetic Characterization of 84882-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84882-5-RR against Human BCL3 were performed. The affinity constant is 0.581 nM.

BCL3 Recombinant monoclonal antibody

BCL3 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84882-5-RR
Clone No.242233B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

242233B11, B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84882-5-RR targets BCL3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13375

Product name: Recombinant human BCL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-446 aa of BC064993

Sequence: NLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 3
Calculated molecular weight 446 aa, 47 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC064993
Gene Symbol BCL3
Gene ID (NCBI) 602
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP20749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain (PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248).

Protocols

Product Specific Protocols
IF protocol for BCL3 antibody 84882-5-RRDownload protocol
IHC protocol for BCL3 antibody 84882-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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